Consensus sequence for HMG1-like DNA binding domains
نویسندگان
چکیده
منابع مشابه
Consensus sequence for HMG1-like DNA binding domains.
Jantzen etal.(l) recently described the sequence of human hUBF, a sequence-specific DNA-binding protein required to activate ribosomal RNA gene transcription. The deduced protein sequence of hUBF contained three domains, called HMG Boxes 1, 2 and 3, each of which shared >35% identity with the DNA-binding domain in the middle of the chromatin-associated High Mobility Group protein 1 (HMG1) (2). ...
متن کاملThe mechanism of sequence non-specific DNA binding of HMG1/2-box B in HMG1 with DNA.
The DNA binding mechanism of box B in HMG1, a member of the sequence non-specific DNA binding HMG1/2-box family of proteins, has been examined by both mutation analyses and molecular modeling techniques. Substitution of the residue 102F, which is characteristically exposed to solvent, with a small hydrophobic amino acid affected its DNA binding activity. However, no additional effect was observ...
متن کاملHMG1 and 2: architectural DNA-binding proteins.
HMG1 and 2 (high mobility group proteins 1 and 2; renamed HMGB1 and 2) contain two DNA-binding HMG-box domains (A and B) and a long acidic C-terminal domain. They bind DNA without sequence specificity, but have a high affinity for bent or distorted DNA, and bend linear DNA. The individual A and B boxes (which, although broadly similar, show both structural and functional differences) exhibit ma...
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Lrp (leucine-responsive regulatory protein) is a major regulatory protein involved in the expression of numerous operons in Escherichia coli. For ilvIH, one of the operons positively regulated by Lrp, Lrp binds to multiple sites upstream of the transcriptional start site and activates transcription. An alignment of 12 Lrp binding sites within ilvIH DNA from two different organisms revealed a te...
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In this study, the interaction of the anthracycline antibiotic doxorubicin with DNA-HMG 1 complex was investigated employing UV/VIS spectroscopy, thermal denaturation and DNA cellulose chromatography techniques. The results indicated that the binding of doxorubicin to the protein reduces its Tm in a dose dependent manner. Although doxorubicin protects free DNA against thermal denaturation ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.18.5565